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Objective To detect hs‐cTnT and cTnI for exploring their application value in the early diagnosis of acute myocardial infarction(AMI) .Methods From October 2015 to March 2016 ,100 patients with AMI in the cardiology department of our hospital were selected as the observation group(AMI group) and contemporaneous 100 adults undergoing physical examination as the con‐trol group .The chemiluminescence immunoassay was used to detect the levels of hs‐cTnT and cTnI .The positive rate ,sensitivity and specificity of the hs‐cTnT and cTnI in the AMI group for early diagnosis of AMI were compared .Results The level of hs‐cT‐nT in the AMI group was (4 .89 ± 1 .83)ng/mL ,which was higher than that in the healthy population by 99 percentile value 0 .014 ng/mL ;the cTnI level in the AMI group was (28 .82 ± 12 .32)ng/mL ,which was higher than that in the healthy population by the upper limit of normal reference value 0 .4 ng/mL ,both of them were significantly higher than those in the control group ( P<0 .05) ,the positive rate and sensitivity of hs‐cTnT was both 92 .0% ,which was significantly higher than 79 .0% of cTnI both(P<0 .05) ,the specificity of cTnI was 96 .0% ,which was significantly higher than 76 .0% of hs‐cTnT(P<0 .05) .Conclusion The pos‐itive rate and the sensitivity of hs‐cTnT are high ,the specificity of cTnI is high ,their joint detection has an important significance for the early diagnosis of AMI .
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Objective To explore the application value of serum procalcitonin (PCT ) and amyloid protein A (SAA ) in the early diagnosis and curative evaluation of bacterial infectious diseases among preschool children in order to improve the clinical diagnosis level of early bacterial infection .Methods A total of 120 cases of pediatric infectious diseases in our hospital from January to De‐cember 2015 were selected and divided into the bacterial infection group (60 cases) and viral infection group (60 cases) ,meanwhile 50 children undergoing the physical examination were selected as the control group .The serum levels of PCT and SAA were ob‐served and compared among the three groups ,and the statistical analysis was performed by using the SPSS 19 .0 software .Results The levels of PCT and SAA in the bacterial infection group were significantly increased compared with the viral infection group and the healthy control group(P<0 .05) ,both PCT and SAA levels after effective antibiotic treatment were significantly decreased (P<0 .05);the positive rates of PCT and SAA in the bacterial infection group were 91 .7% and 95 .0% respectively ,which were signifi‐cantly higher than those in the viral infection group and the healthy control group (P<0 .05) ,the sensitivity of PCT in the diagno‐sis of bacterial infection was lower than that of SAA ,but the specificity ,positive predictive value and negative predictive value were all higher than those of SAA ,the correlation analysis showed that PCT level in the bacterial infection group was positively correla‐ted with and the SAA level(r=0 .782 ,P<0 .01) .Conclusion Detecting the PCT and SAA levels is conducive to early diagnosis , judgment of the disease condition and guidance of rational medication in bacterial infection among preschool children ,in which the efficiency of PCT for diagnosing bacterial infection is superior to SAA .
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Objective To investigate the diagnostic value of combined detection of neutrophil CD64 index and C reactive protein (CRP) levels in newborn bacterial infectious diseases .Methods The retrospective analysis was performed on 139 neonates admitted to the neonatal department of our hospital from January to December 2015 ,including 72 cases in the bacterial infection group and 67 cases in the viral infection group ,and 50 healthy neonates were selected as the control group .The levels of CD64 and CRP and the positive rates were compared among 3 groups and analyzed .The sensitivity ,specificity ,positive predictive value and negative predictive value of CD64 and CRP in the diagnosis of bacterial infection were analyzed .Results The CD64 index and CRP levels had statistical difference between the bacterial infection with the viral infection group and normal control group (P<0 .05) ,the CD64 index and CRP levels after treatment in the bacterial infection group were significantly decreased ,the differences were statistieally significant(P<0 .05) .The sensitivity ,specificity ,positive predictive value and negative predictive value of CD64 index in the diag‐nosis of bacterial infection were 91 .7% ,92 .5% ,93 .0% and 91 .2% respectively ,which were significantly higher than those of CRP ,the differences were statistically significant (P< 0 .05).CD64 index was positively correlated with CRP (r= 0 .781 ,P<0 .01) .Conclusion The combined detection of CD64 index and CRP level helps to early diagnosis ,differential diagnosis and the therapeutic effect evaluation of bacterial infection in neonates ,moreover the diagnostic efficiency of CD64 is obviously better than that of CRP .
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Objective To study and investigate the detection value of noninvasive cerebral hemodynamics and serum nerve func‐tion indexes in the patients with craniocerebral injury .Methods Totally 64 patients with craniocerebral injury in our hospital from September 2013 to May 2015 were selected as the observation group ,meanwhile 64 healthy persons with the same age were selected as the control group .Then the noninvasive cerebral hemodynamics and serum nerve function indexes were compared between the two groups ,furthermore the detection results in the observation group were compared among the patients with different severity de‐grees and intracranial pressures .Results The middle cerebral artery blood flow indexes of the observation group were all higher than those of the control group ,the serum nerve function indexes were also higher than those of the control group ,and the middle cerebral artery blood flow indexes and serum nerve function indexes of the observation group had obvious differences among the pa‐tients with different severity degrees and intracranial pressures too (P< 0 .05) ,showing statistical significance .Conclusion The noninvasive cerebral hemodynamics and serum nerve function indexes have highe detection value in the patients with craniocerebral injury ,and have active clinical role for the understand of disease severity degree and intracranial pressure situation .
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Objective To observe the interference and influence of sample hemolysis on biochemical test results and to make cor-responding countermeasures based on the research results .Methods There were 58 cases of people who underwent physical exami-nation in the hospital .They were selected as study objects .Sample of venous blood was 5 mL in fasting .After natural coagulation and centrifugation ,K+ ,Na+ in blood were measured ,followed by the TP ,CK ,CK-MB ,AST ,ALB ,TG ,HDL ,LDH ,HBDH and other biochemical indexes .Then all indexes mentioned above were detected again after the sample hemolysis of serum ,and analysis results were compared between them .Results Of biochemical indicators detected before and after the determination of hemolysis , there were of statistical significance in the differences in K + ,Na+ ,TP ,CK ,CK-MB ,AST ,LDH and HBDH(P0 .05) .Through regression analysis ,biochemi-cal indicators such as K + ,Na+ ,TP ,CK ,CK-MB ,AST ,LDH and HBDH were found to be related to hemolysis .Conclusion Sample hemolysis has certain influences on the results of biochemical test in terms of K + ,Na+ ,TP ,CK ,CK-MB ,AST ,LDH and HBDH , which is of certain application value with the proofreading of serum Hb concentration .
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Objective To investigate the expression and function of Fca receptor on human airway smooth muscle(ASM)cells.Methods RT-PCR and immunofluorescence technique were used to detect the expression of Fca receptor on human ASM cells.The effect of IgA on intracellular calcium concentration of human ASM cells was measured by using Fura-2/AM as a calcium indicator.Results RT-PCR and immunofluorescence staining confirmed the presence of Fca receptor on human ASM cells.Compared with control,secretory IgA(sIgA)induced a rise in intracellular calcium concentrations on human ASM cells after 90min incubation(P0.05).Conclusion Fca receptor was expressed on human ASM cells.SIgA increased the intracellular calcium concentration of human ASM cells.
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OBJECTIVE: To observe the influence of arecoline (Ar) on human sperm motility in vitro. METHODS: 50 cases of normal human male sperm which had been screened were incubated in different concentration of Ar solution (10 ?g?mL-1,50 ?g?mL-1,100 ?g?mL-1),taking the optimized sperm as control group. After the incubation lasting 0.5,1,2 h, computer assisting sperm analysis system (CASA) was used to analyze the Mot, (a+b) PM,VCL and VSL of sperm. RESULTS: The Mot of sperm incubated in 10 ?g?mL-1 Ar solution for 1 h was significantly different from that of control group(P